User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/03/09

From OpenWetWare
Jump to navigationJump to search
Applied Soil Microbial Ecology <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Remains of the Day

  • Do real time PCR on potato cDNA samples from Jan 18 that were made yesterday
  • Qiagen RNeasy kit arrived - use it next week
  • Begin all soil treatments (weight assumed to be 200 kg/m^2 - all % mixtures are weight or volume product:weight dry soil - amendmants will be applied in 1 L of water mixed with fertilizer to 1 kg of soil) for tomato amendmant rhizosphere trial including treatments with 5 plants each
    • Autoclaved sand
    • Negative control (just water and initial fertilization)
    • Pasteurized (75 degree oven for 12 hours)
    • Terrabiogen liquid 1%
    • Terrabiogen liquid 5%
    • Terrabiogen solid 1%
    • Biofence brassica meal 0.2%
    • OmegaGrow Fish Emulsion 3% (based on volume)
    • Molasses 1% (based on volume)
    • Azospirrilum and Strenotrophomonas dip and drench (each at OD600=0.2 in water)