User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/10/12

From OpenWetWare
Jump to navigationJump to search
Maize Endophyte Biofertilizers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

The Daily Grind

  • tested out cDNA and RT pcr primers for potatoe and tomatoe PR1 and EF1. Annealing was 56 Celcius, 30 second extention time with 35 cycles.



  • the cDNA all came out great, but I think I forgot to add taq to the PR1 tests on the bottom row (far left bottom is negative control followede by potato pr1, tomatoe pr1, then left over + ef1, and blended ef1)