User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/22: Difference between revisions
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* Did not detect D-luc signal. Perhaps D-luc imaging should start immediately after adding reagents. The D-luc signal has a short half-life. | * Did not detect D-luc signal. Perhaps D-luc imaging should start immediately after adding reagents. The D-luc signal has a short half-life. | ||
* Cells rounded up a bit after sitting in PBS. Next time use 10% FBS in PBS, or try DMEM without red dye. | * Cells rounded up a bit after sitting in PBS. Next time use 10% FBS in PBS, or try DMEM without red dye. | ||
* DAPI signal was barely visible | |||
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* HEK293 Gal4-EED, 1:4 | * HEK293 Gal4-EED, 1:4 | ||
* HEK293 Gal4-EED, 1:4 in 0.5 μg/mL puromycin (to select for Gal4-EED RNAi tuner) | * HEK293 Gal4-EED, 1:4 in 0.5 μg/mL puromycin (to select for Gal4-EED RNAi tuner) | ||
Revision as of 20:53, 18 March 2013
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02/23/13
DAPI/ luciferase staining
Used 3 wells:
Results/ Conclusions:
New cell plates
Stock cultures (T75 flasks)
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