User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/15: Difference between revisions
From OpenWetWare
Line 11: | Line 11: | ||
---- | ---- | ||
''HEK293-luc #4 cell culturing, trial 2'' | '''HEK293-luc #4 cell culturing, trial 2''' | ||
* Tried plating at the optimal density (1600 cells/well) in a 6-well plate; cells did not grow very well | * Tried plating at the optimal density (1600 cells/well) in a 6-well plate; cells did not grow very well | ||
Line 19: | Line 19: | ||
* Made the following dilutions: | * Made the following dilutions: | ||
**(1) 1:25 dilution of harvested cells = 40,000 cells/mL 10% FBS DMEM | **(1) 1:25 dilution of harvested cells (1mL cells + 24 mL medium) = 40,000 cells/mL 10% FBS DMEM | ||
**(2) 1:25 dilution of tube 1 = 1:625 dilution = 1,600 cells/mL 10% FBS DMEM | **(2) 1:25 dilution of previous tube (1mL 1:25 cells + 24 mL medium) = 1:625 dilution = 1,600 cells/mL 10% FBS DMEM | ||
**(3) 1:100 dilution of | **(3) 1:100 dilution of harvested cells --> 1:100 dilution of these | ||
# 6-well plate: 2 mL , 80,000 cells/ well | # 6-well plate: 2 mL of 1:25 dln., ~80,000 cells/ well | ||
# 6-well plate: 2 mL | # 6-well plate: 2 mL of 1:625 dln., ~3,200 cells/ well | ||
# 96-well plate: 200 uL of 1:40,000 dln., ~5 cells/ well | |||
Revision as of 18:34, 18 February 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
02/15/13
HEK293-luc #4 cell culturing, trial 2
|