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02/06/13
- HEK293-luc14 plate: 6-well plate
HEK293-luc14 plate
- Plated HEK293-luc14 positive control cells at optimal density (1.6x103), as determined by previous dilution plating
- Tryspinized cells, brought conc. to 1x106</up>/mL
- Added 0.5 mL to 12 mL medium (1/25 dilution)
- Added 0.5 mL of 1/25 dilution to 12 mL medium (1/625 dilution)
- Added 1 mL of the 1/625 dilution to each well
- Added an additional 1 mL growth medium to each well (to prevent the medium from drying out)
- Grow for 7 days to get colonies
- Will stain with Hoescht (DAPI) for fluorescent imaging; maybe try D-luciferin when it arrives.
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