User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/06
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| - | == | + | ==02/06/13== |
| - | * | + | * HEK293-luc14 plate: 6-well plate |
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| + | '''HEK293-luc14 plate''' | ||
| + | * Plated HEK293-luc14 positive control cells at optimal density (1.6x10<sup>3</sup>), as determined by previous dilution plating | ||
| + | ** Tryspinized cells, brought conc. to 1x10<sup>6</up>/mL | ||
| + | ** Added 0.5 mL to 12 mL medium (1/25 dilution) | ||
| + | ** Added 0.5 mL of 1/25 dilution to 12 mL medium (1/625 dilution) | ||
| + | ** Added 1 mL of the 1/625 dilution to each well | ||
| + | ** Added an additional 1 mL growth medium to each well (to prevent the medium from drying out) | ||
| + | * Grow for 7 days to get colonies | ||
| + | * Will stain with Hoescht (DAPI) for fluorescent imaging; maybe try D-luciferin when it arrives. | ||
Revision as of 22:33, 6 February 2013
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02/06/13
HEK293-luc14 plate
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