User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/06: Difference between revisions
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'''HEK293-luc14 plate''' | '''HEK293-luc14 plate''' | ||
* Plated HEK293-luc14 positive control cells at optimal density (1.6x10<sup>3</sup>), as determined by previous dilution plating | * Plated HEK293-luc14 positive control cells at optimal density (1.6x10<sup>3</sup>), as determined by previous dilution plating | ||
** Tryspinized cells, brought conc. to 1x10<sup>6</ | ** Tryspinized cells, brought conc. to 1x10<sup>6</sup>/mL | ||
** Added 0.5 mL to 12 mL medium (1/25 dilution) | ** Added 0.5 mL to 12 mL medium (1/25 dilution) | ||
** Added 0.5 mL of 1/25 dilution to 12 mL medium (1/625 dilution) | ** Added 0.5 mL of 1/25 dilution to 12 mL medium (1/625 dilution) |
Revision as of 19:34, 6 February 2013
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02/06/13
HEK293-luc14 plate
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