User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/01/25 Entry for User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern)
(Entry title)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Preparation of a dilution sequence==
-
* Insert content here...
+
To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.
 +
Cell line: HEK 293-Luc14
 +
Plates: 6-well plates
 +
Dilution rate: 1:5
 +
Starting with a full grown plate mixed into 10ml liquid.
 +
Well No. : Approximate avg. cell count
 +
1:  1,000,000
 +
2:  200,000
 +
3:  40,000
 +
4:  8,000
 +
5:  1,600
 +
6:  320
 +
7:  64
 +
8:  ~12
 +
9:  ~2
 +
10:<1
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 14:08, 30 January 2013

Project name Main project page
Next entry

Preparation of a dilution sequence

To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. Cell line: HEK 293-Luc14 Plates: 6-well plates Dilution rate: 1:5 Starting with a full grown plate mixed into 10ml liquid.

Well No. : Approximate avg. cell count 1: 1,000,000 2: 200,000 3: 40,000 4: 8,000 5: 1,600 6: 320 7: 64 8: ~12 9: ~2 10:<1


Personal tools