User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25

Preparation of a dilution sequence

To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.

Cell line: HEK 293-Luc14

Plates: 6-well plates

Dilution rate: 1:5

Starting with a full grown plate mixed into 10ml liquid.

Well No. : Approximate avg. cell count

1: 1,000,000

2: 200,000

3: 40,000

4: 8,000

5: 1,600

6: 320

7: 64

8: ~12

9: ~2

10:<1