User:David Benjamin Nyer/Notebook/PcTF breast cancer/2016/06/23: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PcTF breast cancer</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PcTF breast cancer</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Summary==
==Summary==


Summarize what happened today. Put methods, data, and next steps in sections below.
Polymer transfection results: none of the 12 samples show high levels of fluorescence. Transfection efficiency appears to be very low regardless of polymer type or concentration. Massive amount of cell death in higher polymer concentration samples. Images were taken 24 hours post transfection.
 
==AGC18 (1:5)==
 
10:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_1_AGC18_1-5_KAH126-MV2_10_1_phase_mCherry_10x.jpg | 500px]]
 
25:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_2_AGC18_1-5_KAH126-MV2_25_1_phase_mCherry_10x.jpg | 500px]]
 
50:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_3_AGC18_1-5_KAH126-MV2_50_1_phase_mCherry_10x.jpg | 500px]]
 
==NGC18 (1:5)==
 
10:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_4_NGC18_1-5_KAH126-MV2_10_1_phase_mCherry_10x.jpg | 500px]]
 
25:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_5_NGC18_1-5_KAH126-MV2_25_1_phase_mCherry_10x.jpg | 500px]]
 
50:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_6_NGC18_1-5_KAH126-MV2_50_1_phase_mCherry_10x.jpg | 500px]]
 
==NR==
 
10:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_7_NR_KAH126-MV2_10_1_phase_mCherry_10x.jpg | 500px]]
 
25:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_8_NR_KAH126-MV2_25_1_phase_mCherry_10x.jpg | 500px]]
 
50:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_9_NR_KAH126-MV2_50_1_phase_mCherry_10x.jpg | 500px]]
 
==PGC18 (1:5)==
 
10:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_10_PGC18_1-5_KAH126-MV2_10_1_phase_mCherry_10x.jpg | 500px]]
 
25:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_11_PGC18_1-5_KAH126-MV2_25_1_phase_mCherry_10x.jpg | 500px]]
 
50:1 polymer:DNA<br>
[[Image:2016-06-23_MCF10A_Well_12_PGC18_1-5_KAH126-MV2_50_1_phase_mCherry_10x.jpg | 500px]]





Latest revision as of 01:49, 27 September 2017

PcTF breast cancer Main project page
Previous entry      

Summary

Polymer transfection results: none of the 12 samples show high levels of fluorescence. Transfection efficiency appears to be very low regardless of polymer type or concentration. Massive amount of cell death in higher polymer concentration samples. Images were taken 24 hours post transfection.

AGC18 (1:5)

10:1 polymer:DNA

25:1 polymer:DNA

50:1 polymer:DNA

NGC18 (1:5)

10:1 polymer:DNA

25:1 polymer:DNA

50:1 polymer:DNA

NR

10:1 polymer:DNA

25:1 polymer:DNA

50:1 polymer:DNA

PGC18 (1:5)

10:1 polymer:DNA

25:1 polymer:DNA

50:1 polymer:DNA