User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/25: Difference between revisions
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==Summary== | ==Summary== | ||
BT-474 transfection results and next steps. | |||
==BT-474 transfection results== | |||
Transfection efficiency very poor throughout, based on fluorescence microscopy. Not going to worry about harvesting and measuring efficiency via flow cytometry. | |||
Next steps: set up two 6-well plates per cell type with 3 cultures per plate. Try these transfection optimization steps:<br> | |||
# Transfection followed by 30 minutes centrifugation at 100rpm; | |||
# Trypsinization and resuspension in fresh media, followed by transfection so that cells are non-adherent and floating around in lipo-DNA saturated media. | |||
Revision as of 12:12, 25 November 2015
 PcTF breast cancer
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SummaryBT-474 transfection results and next steps. BT-474 transfection resultsTransfection efficiency very poor throughout, based on fluorescence microscopy. Not going to worry about harvesting and measuring efficiency via flow cytometry. Next steps: set up two 6-well plates per cell type with 3 cultures per plate. Try these transfection optimization steps:
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