User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/24: Difference between revisions
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==Summary== | ==Summary== | ||
Imaged BT-549 transfection, started BT-474 transfection. | |||
==BT-549 transfection results== | |||
Transfection was very poor, probably due to high culture density. Not going to worry about posting results, will repeat with lower confluency. One note: highest concentration of LTX reagent (15 µL) appears to be most effective. | |||
==BT-474 transfection setup== | |||
Same as yesterday's transfection, but with BT-474 cell line instead. Identical procedure. | |||
Revision as of 17:28, 24 November 2015
 PcTF breast cancer
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SummaryImaged BT-549 transfection, started BT-474 transfection. BT-549 transfection resultsTransfection was very poor, probably due to high culture density. Not going to worry about posting results, will repeat with lower confluency. One note: highest concentration of LTX reagent (15 µL) appears to be most effective. BT-474 transfection setupSame as yesterday's transfection, but with BT-474 cell line instead. Identical procedure.
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