User:DavidSavage
Biography
I was born and raised in Mt. Pleasant, Iowa, a smallish farming town in the southeast corner of the state. As an undergraduate I studied chemistry and CS at Gustavus Adolphus College and also ran on the track and cross country teams. Following college I moved out west and spent several years exploring California, eating through San Francisco, and studying membrane protein structure and function in the lab of Bob Stroud at UCSF. I currently live in Cambridge, MA and am a postdoctoral fellow with Pamela Silver. Outside of the lab, I enjoy spending time with Sarah, running, cooking, snowboarding, and gardening.
Research Interests
I am broadly interested in better defining the biological reactions of photosynthesis. This process is of singular importance to humanity and poses many interesting questions that span from atomic to ecological levels. I am currently developing a cyanobacterial chassis as the basis for experiments in protein structure and engineering, elucidating gene networks (in collaboration with Ron Milo), and of metabolic engineering for the production of biofuels.
Publications
12. Engineering of the aquaporin selectivity filter. Savage, D.F., O’Connell III, J.D., Stroud R.M. (2010) In press at PNAS.
11. Engineered synthesis and export of hydrophillic products from cyanobacteria. Niederholt meyer, H., Wolfstaedter, B., Savage, D.F., Silver, P., Way, J.C. (2010) Appl. Environ. Microbiol, 76:3462-6. [PDF]
10. Spatially ordered dynamics of the bacterial carbon fixation machinery. Savage, D.F., Afonso, B., Chen, A., Silver, P.A. (2010) Science, 327:1258-61. [[media: |[PDF]]]
9. A general protocol for the crystallization of membrane proteins for X-ray structural investi gation. Newby, Z.E., O'Connell 3rd, J.D., Gruswitz, F., Hays, F.A., Harries, W.E., Harwood, I.M., Ho, J.D., Lee, J.K., Savage, D.F., Miercke, L.J., Stroud, R.M. (2009) Nat Protoc. 4:619-37. [PDF]
8. Defossiling fuel: How synthetic biology can transform biofuel production. Savage, D.F., Way, J., Silver, P.A. (2008) ACS Chem. Biol. 3:13-6. [PDF]
7. Structural basis of aquaporin inhibition by mercury. Savage, D.F. and Stroud, R.M. (2007) J. Mol. Biol. 368: 607-17. [PDF]
6. Cell-free complements in vivo expression of the E. coli membrane proteome. Savage, D.F., Anderson C.L, Robles-Colmenares, Y., Newby, Z.A., Stroud R.M. (2007) Protein Sci. 16:966-76. [PDF]
5. Substrate twinning activates the signal recognition particle and its receptor. Egea P.F., Shan S.O., Napetschnig J., Savage D.F., Walter P., Stroud R.M. (2004) Nature, 426:215-221. [PDF]
4. Water and glycerol permeation through the glycerol channel GlpF and the aquaporin family. Lee, J.K., Khademi, S., Harries, W., Savage, D., Miercke, L, Stroud, R.M. (2004) J. Synchrotron Radiat. 11:86-88
3. Architecture and selectivity in aquaporins: 2.5Å x-ray structure of aquaporin Z. Savage, D. F., Egea, P.F., Robles, Y.C., O’Connell III, J.D., and Stroud, R.M. (2003) PLoS Biology, 1:334-340. [PDF]
2. Selectivity and conductance among the glycerol and water conducting aquaporin family of channels. Stroud, R.M., Savage, D.F., Miercke, L.J., Lee, J.K., Khademi, S., Harries, W. (2003). FEBS Letters, 555:79-84. [PDF]
1. Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA: ACP transacylase. Keatinge-Clay, A.T., Shelat, A.A., Savage, D.F., Tsai, S.C., Miercke, L.J.W., O'Connell 3rd, J.D., Khosla, C., and Stroud, R.M. (2003) Structure, 11:147-154. [PDF]
Contact info
Dave Savage
Department of Systems Biology
Harvard Medical School
200 Longwood Ave. WAB 536
Boston, MA 02115
T 617.432.6402
C 415.637.4450
