User:Darrell A Humphries Jr/Notebook/Biology 210 at AU: Difference between revisions
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4/4 | 4/4 Exercise III Microbiology and Identifying Bacteria | ||
The state of the Hay Infusion Culture me and my lab partners observed, show notable physical change from the first time the Hay Infusion Culture were observed. A stronger, darker odor is emitting from its container (sewer-like), while evidence of more bacteria growth have become present. The turbidity of the water has increased, it has more difficult to see through the water as it is much more 'cloudy' than before, possibly due to an increase in particles and bacteria. | |||
Purpose: The purpose of this lab is to identify species of bacteria based on motility, gram stain, colony morphology and sequencing of the 16s ribosomal subunit gene. | |||
Methods & Materials: Using the Agar plates inoculated from the hay infusion culture, observation and quantifying present microorganisms with use of a microscope and the naked eye, we were able to count the total numbers of colonies on each plate. The Prokaryotes are observed with a microscope because they range in small size from 1 to 10 um. The Wet mount procedure and Gram stain procedure were used to determine the cell shapes and if the organisms are motile, and determination of gram-positive vs gram-negative bacteria. | |||
Results: The state of the Hay Infusion Culture me and my lab partners observed, show notable physical change from the first time the Hay Infusion Culture were observed. A stronger, darker odor is emitting from its container (sewer-like), while evidence of more bacteria growth have become present. The turbidity of the water has increased, it has more difficult to see through the water as it is much more 'cloudy' than before, possibly due to an increase in particles and bacteria. | |||
Dilution |Agar Type | # Colonies on plate | Conversion Factor | Colonies/mL | | Dilution |Agar Type | # Colonies on plate | Conversion Factor | Colonies/mL | | ||
Revision as of 12:17, 11 February 2016
4/4 Exercise III Microbiology and Identifying Bacteria
Purpose: The purpose of this lab is to identify species of bacteria based on motility, gram stain, colony morphology and sequencing of the 16s ribosomal subunit gene.
Methods & Materials: Using the Agar plates inoculated from the hay infusion culture, observation and quantifying present microorganisms with use of a microscope and the naked eye, we were able to count the total numbers of colonies on each plate. The Prokaryotes are observed with a microscope because they range in small size from 1 to 10 um. The Wet mount procedure and Gram stain procedure were used to determine the cell shapes and if the organisms are motile, and determination of gram-positive vs gram-negative bacteria.
Results: The state of the Hay Infusion Culture me and my lab partners observed, show notable physical change from the first time the Hay Infusion Culture were observed. A stronger, darker odor is emitting from its container (sewer-like), while evidence of more bacteria growth have become present. The turbidity of the water has increased, it has more difficult to see through the water as it is much more 'cloudy' than before, possibly due to an increase in particles and bacteria.
Dilution |Agar Type | # Colonies on plate | Conversion Factor | Colonies/mL |
10^-3 |nutrient | 756 | x10^3 | 756,000 |
10^-5 |nutrient | 64 | x10^5 | 6,400,000 |
10^-7 |nutrient | 2 | x10^7 | 20,000,000 |
10^-9 |nutrient | 0 | x10^9 | 0 |
10^-3 |nutrient + tet | 70 | x10^3 | 70,000 |
10^-5 |nutrient + tet | 0 | x10^5 | 0 |
10^-7 |nutrient + tet | 0 | x10^7 | 0 |
10^-9 |nutrient + tet | 0 | x10^9 | 0 |
- This table indicates that the antibiotic Tetracycline was effective with inhibiting bacteria growth on the Agar Plates; so effective that our last three(3) agar plates recorded zero(0) bacteria colonies on the Agar Plate. Dilution 10^-7 without the tetracycline recorded 20 million colonies/mL of bacteria, while dilution 10^-7 with tetracycline recorded zero(0) colonies/mL of bacteria.
Mechanisms of action for tetracycline:Citation -> Antimicrobial Research Centre and Division of Microbiology, School of Biochemistry & Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom,1 and Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98195-72382 <- \Mechanisms of action for tetracycline/
- inhibit protein synthesis by preventing the attachment of aminoacyl-tRNA to the ribosomal acceptor (A) site
Types of Bacteria effected:
- Tetracyclines are broad-spectrum agents, exhibiting activity against a wide range of gram-positive and gram-negative bacteria, atypical organisms such as chlamydiae, mycoplasmas, and rickettsiae, and protozoan parasites.
Colony Label | Plate Type| Colony Description + Cell Description | Gram + or Gram - | Additional Notes |
1 | - | Orange, white like | |
pigments. Circular & irregular. | negative |
Flat & raised elevation. Filamentous | |
Colony. | |
2 | - | Yellow, orange, white, smooth, | |
some convex elevation. Rhizoid | positive |
colony and circular | |
3 | + | Mixture of circular, irregular | |
& filamintous colonies. Lobat & | positive | gram positive 1um- pink circular bacteria
entire edge. Convex elevation, | |
smooth surface, jell-like. | |
4 | + | No Bacteria Growth | negative |
Media:https://scontent-lga3-1.xx.fbcdn.net/hphotos-xft1/v/t34.0-12/12654522_10200225055808888_4943828854081192355_n.jpg?oh=c7452ace4a24d4962cb0c40a68df8b14&oe=56B578F3
Here in this image of colony label,there is a mixture of circular, irregular and filamentous colonies. Yellow, orange, white.
