User:Danyan Tang/Notebook/Biology 210 at AU

Lab 2. identifying algae and protists Jan 28 2015 Purpose: To identify different unknown organisms by using a dichotomous key and use this to understand the characteristics of protists and algae. Also to identify protists and algae in the Hay infusion Culture was made from our assigned transect.

Material and methods: 1.Observed a wet mount of known organisms under microscope and used the dichotomous key to identify them. 2.To observe our culture by taking from 2 different niches,we took them from top and bottom separately.Then made a wet mount for each of the samples and identified protist and algae from the Hay Infusion Culture by using the dichotomous key. 3.prepared a total of eight petri dishes by using sterile broth,mixture of Hay infusion culture,nutrient agar and nutrient agar plus tetracycline.Placed all of them at room temperature and will use them for lab 3.

Data and observation: when we observed the Hay infusion culture we made last week, we could smell the water likes stinking sewers and it started to evaporate. We could see the moss and soil were in the bottom and there were a couple of berries and wood on the top of muddy and turbid water. There is no apparent life like molds or green shoots on the top the liquid.(#1&2) We took samples from the top and bottom, colpidium and gonium were found from the top and also found colpidium from bottom. Gonium was about 20µm, appears in green color with disc-shaped cells.(#3&5) Colpidium 25µm, appears colorless and oval-shaped. It exhibited motility because of cilia.(#4&5) Prepare for lab 3. (#6)

1. 2. 3. photo by Corina.C 4. Photo by Corina.C 5. 6.

Conclusion and future direction: After lab 2, we understand how to identify different organisms using the dichotomous key. However, only one week is not enough time let the our jar to grow, if we put the culture for a long time period they might reproduce the we can find our more organisms. In the future, we can supply our culture some nutrient in order to keep them for future investigation.

A.T

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1.27.15 Very good first entry. Try to use past tense and describe what you did rather than what was in the lab manual instructions. Try to rotate the photos if possible. SK

Lab 1. Biological life at AU Jan 15 2015 Purpose: 1.Observe 3 types of green algae using microscope and depression slides and understand how natural selection leads the evolution. 2. To observe and analyze a assigned transect at the AU campus.We will use the biotic and abiotic as samples to examine through the serval weeks for this semester and discuss the relationships between species in ecosystem.

Material and methods:1.(for the first part of lab) observe 3 types of green algae under the microscope which were chlamydomonas, gonium and volvox. Analyze them and fill out the information on the notebook. 2.(for the second part of lab) The TA gave each one a number to form a group of three assigned to each transect. our group went to the transect to take the sample with a 50mL conical tube.Back to the lab, we weighed out 11.22g of soil sample transferred them to a jar and added 500ml Deerpark water and 0.14g dried milk powder to make Hay infusion culture. Mix the jar for 10 second and placed them without lid for next week.

Data and observation:1.examine 3 green algae group,chlorophyta--the volvocine line. (#1)2. The 20 by 20 meter assigned transect was located on the North side of campus on the side where Hughes Hall and McDowell Hall are adjacent to each other.(#2) There are some abiotic and biotic components. abiotic:rocks, sunlight, shade from building,bench, soil and snow. biotic: tree, moss bushes and berries. (#3-#7)

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2. 2. photo by Corina C.Velazco
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Conclusions and Future Directions: There are some condition can affect the transect, like the components, weather and so on. By using the Hay infusion culture, we can observe protists and study different species of bacteria. This can help us to understand how natural selection leads the evolution of different species. AT