User:Daniel Goodman/Notebook/Weiss/2010/01/19: Difference between revisions
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| 2 || PIF3 || ~15ul || 1.5ul | | 2 || PIF3 || ~15ul || 1.5ul | ||
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| 3- | | 3 || PIF3 Old || ~15ul || 1.5ul | ||
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| 4-10 || PhyB917 Samples 1-7* || ~15ul || 1.5ul | |||
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''* Samples 1-3 are new, 4-7 are recovered from freezer from Dec, may fail'' | |||
===Expected for PhyB917:=== | ===Expected for PhyB917:=== |
Revision as of 12:05, 19 January 2010
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> | ||||||||||||||||||||
Restriction and Gel of PhyB917 & PIF3Samples First 3 PhyB917 Samples (37 deg C, 10 min) PIF 3 Sample (65 deg C, 10 min) Made 75 ml agar gel:
Put SYBR after cooling, cooled in water bath, set gel ~20 min. Note: forgot to swirl after adding sybr, though color looked uniform Cut top of gel off, saved bottom in 4°C fridge in ziploc w/ name&date Loading Gel:
* Samples 1-3 are new, 4-7 are recovered from freezer from Dec, may fail Expected for PhyB917:Cut with BstBI - two bands, 3579 and 5314 Expected for PIF3:Cut with AvrII - two bands, 2164 and 4791
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