User:Daniel Goodman/Notebook/Weiss/2010/01/14: Difference between revisions
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|Concentration of DNA ng/ul||400 | |||
Concentration of DNA ng/ul||400 | |Concentration of enzyme Units/ul||20 | ||
| | |Overdigestion factor||2 | ||
Concentration of enzyme Units/ul||20 | |Volume of DNA / ul||0.5 | ||
| | |Volume of enzyme / ul||0.02 | ||
Overdigestion factor||2 | |Calculate Total Volume||0.5 | ||
| | |Actual Total Volume||15 | ||
Volume of DNA / ul||0.5 | |NEB Buffer||1.5 | ||
| | |Water||12.98 | ||
Volume of enzyme / ul||0.02 | |BSA||0 | ||
| | |Water||12.98 | ||
Calculate Total Volume||0.5 | |ENZYME||0.145 | ||
| | |BUFFER(4)||10.875 | ||
Actual Total Volume||15 | |WATER||94.105 | ||
| | |BSA||0 (none req'd for BstBII) | ||
NEB Buffer||1.5 | |TOAL VOL PER ALIQ||14.5 | ||
| | |(add 0.5 of dna to each) | ||
Water||12.98 | |} | ||
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BSA||0 | |||
| | |||
Water||12.98 | |||
ENZYME 0.145 | |||
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BUFFER(4) 10.875 | |||
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WATER 94.105 | |||
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BSA 0 (none req'd for BstBII) | |||
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TOAL VOL PER ALIQ | |||
| | |||
(add 0.5 of dna to each) | |||
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Revision as of 09:13, 14 January 2010
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Entry titleWill Run PhyB917, PhyB, and PIF3 on a Gel PhyB917 Cut with BstBI - two bands, 3579 and 5314 incubate at 65 degrees with NEBuffer 4
Taking average, 400 ng/ul Calculate digestion mix for 7.25 aliquots: calculation based on mass of DNA to be digested||
PIF3 (possibly) Cut with AvrII - two bands, 2164 and 4791 incubate at 37 deg w/ N||||EBuffer 4 Nanodrop on PIF3 DNA sample: 357.4 ng/ul One enzyme digest|| calculation based on mass of DNA to be digested|| Mass of DNA / ug||0.2 Units of enzyme||0.2 Concentration of DNA ng/ul||350 Concentration of enzyme Units/ul||4 Overdigestion factor||2 Volume of DNA / ul||0.571428571 Volume of enzyme / ul||0.1 Calculate Total Volume||1 Actual Total Volume||15 NEB Buffer||1.5 Water||12.82857143 BSA||0 Water||12.82857143
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