User:Daniel Goodman/Notebook/Cluzel/2010/04/05
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(→PZE-12-CFP vector from JM Kim) |
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{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
|- | |- | ||
| + | ! Symbol | ||
! Media | ! Media | ||
! Plasmid | ! Plasmid | ||
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! Why | ! Why | ||
|- | |- | ||
| + | | A | ||
| LA/Amp | | LA/Amp | ||
| PZE-12-CFP | | PZE-12-CFP | ||
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| To get comp cells w/ plasmid | | To get comp cells w/ plasmid | ||
|- | |- | ||
| + | | B | ||
| LA | | LA | ||
| PZE-12-CFP | | PZE-12-CFP | ||
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| To check that my competent cells are alive | | To check that my competent cells are alive | ||
|- | |- | ||
| + | | C | ||
| Spec/AMP | | Spec/AMP | ||
| PZS4-ATTP | | PZS4-ATTP | ||
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| To check that my competent cells transform | | To check that my competent cells transform | ||
|- | |- | ||
| + | | D | ||
| LA/AMP | | LA/AMP | ||
| PZE-12-CFP | | PZE-12-CFP | ||
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Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book | Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book | ||
| - | * | + | * used straight glycerol instead of GYT |
| - | * | + | * used SOC medium (1 ml per reaction) |
| - | * | + | * 1 ul of DNA per experiment (approx 52 ng for my plasmid, did not nanodrop Jeff's spec plasmid) |
| + | * 1.8 kV, 1 second shock | ||
Revision as of 18:37, 5 April 2010
 Cluzel Lab Notebook Daniel Goodman |  Main project page Previous entry Next entry
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Prepping Plasmid for ElectroporationPZE-12-CFP vector from JM Kim
Doing 4 electroporations, in order to ensure competent cells made last week work correctly.
Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book
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