User:Daniel Goodman/Notebook/Cluzel/2010/04/05

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(Prepping Plasmid for Electroporation)
(PZE-12-CFP vector from JM Kim)
Line 34: Line 34:
|  To check that my competent cells are alive
|  To check that my competent cells are alive
|-
|-
-
LA/AMP
+
Spec/AMP
-
Amp Plasmid (from Jeff)
+
PZS4-ATTP
|  My competent MG1655
|  My competent MG1655
|  To check that my competent cells transform
|  To check that my competent cells transform

Revision as of 16:27, 5 April 2010


Cluzel Lab Notebook
Daniel Goodman
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Prepping Plasmid for Electroporation

PZE-12-CFP vector from JM Kim

ng/ul 260/280 260/230
56.3 ng 1.92 1.62

Doing 4 electroporations, in order to ensure competent cells made last week work correctly.

Media Plasmid Cells Why
LA/Amp PZE-12-CFP My competent MG1655 To get comp cells w/ plasmid
LA PZE-12-CFP My competent MG1655 To check that my competent cells are alive
Spec/AMP PZS4-ATTP My competent MG1655 To check that my competent cells transform
LA/AMP PZE-12-CFP Jeff's competent MG1655 To check that my plasmid works/is correct

Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book

  • need GYT medium
  • need SOC medium (1 ml per reaction)
  • 10 ng of DNA per experiment



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