User:Daniel Goodman/Notebook/Cluzel/2010/04/05

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(Prepping Plasmid for Electroporation)
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Per electroporation, need up to 25 ng of DNA
 
Doing 4 electroporations, in order to ensure competent cells made last week work correctly.
Doing 4 electroporations, in order to ensure competent cells made last week work correctly.
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Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book
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*need GYT medium
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*need SOC medium (1 ml per reaction)
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* 10 ng of DNA per experiment
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Revision as of 13:03, 5 April 2010


Cluzel Lab Notebook
Daniel Goodman
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Prepping Plasmid for Electroporation

PZE-12-CFP vector from JM Kim

ng/ul 260/280 260/230
56.3 ng 1.92 1.62

Doing 4 electroporations, in order to ensure competent cells made last week work correctly.

Media Plasmid Cells Why
LA/Amp PZE-12-CFP My competent MG1655 To get comp cells w/ plasmid
LA PZE-12-CFP My competent MG1655 To check that my competent cells are alive
LA/AMP Amp Plasmid (from Jeff) My competent MG1655 To check that my competent cells transform
LA/AMP PZE-12-CFP Jeff's competent MG1655 To check that my plasmid works/is correct

Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book

  • need GYT medium
  • need SOC medium (1 ml per reaction)
  • 10 ng of DNA per experiment



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