User:Daniel Goodman/Notebook/Cluzel/2010/04/05: Difference between revisions
From OpenWetWare
| Line 14: | Line 14: | ||
| 1.62 | | 1.62 | ||
|} | |} | ||
Doing 4 electroporations, in order to ensure competent cells made last week work correctly. | Doing 4 electroporations, in order to ensure competent cells made last week work correctly. | ||
| Line 47: | Line 45: | ||
|} | |} | ||
Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book | |||
*need GYT medium | |||
*need SOC medium (1 ml per reaction) | |||
* 10 ng of DNA per experiment | |||
|} | |} | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 10:03, 5 April 2010
 Cluzel Lab Notebook Daniel Goodman |
 Main project page Previous entry Next entry <sitesearch>title=Search this Project</sitesearch>
| |||||||||||||||||||||||||||
Prepping Plasmid for ElectroporationPZE-12-CFP vector from JM Kim
Doing 4 electroporations, in order to ensure competent cells made last week work correctly.
Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book
| ||||||||||||||||||||||||||||
