User:Daniel Goodman/Notebook/Cluzel/2010/04/05

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(Prepping Plasmid for Electroporation)
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==Prepping Plasmid for Electroporation==
==Prepping Plasmid for Electroporation==
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*PZE-12-CFP vector from JM Kim
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===PZE-12-CFP vector from JM Kim===
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** Vector Nomenclature: http://www.expressys.com/main_vectors.html
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* Vector Nomenclature: http://www.expressys.com/main_vectors.html
{| class="wikitable" border="1"
{| class="wikitable" border="1"
|-
|-
-
!  ng
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!  ng/ul
!  260/280
!  260/280
!  260/230
!  260/230
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|}
|}
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Per electroporation, need up to 25 ng of DNA
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 +
Doing 4 electroporations, in order to ensure competent cells made last week work correctly.
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 +
{| class="wikitable" border="1"
 +
|-
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!  Media
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!  Plasmid
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!  Cells
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!  Why
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|-
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|  LA/Amp
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|  PZE-12-CFP
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|  My competent MG1655
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|  To get comp cells w/ plasmid
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|-
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|  LA
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|  PZE-12-CFP
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|  My competent MG1655
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|  To check that my competent cells are alive
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|-
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|  LA/AMP
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|  Amp Plasmid (from Jeff)
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|  My competent MG1655
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|  To check that my competent cells transform
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|-
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|  LA/AMP
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|  PZE-12-CFP
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|  Jeff's competent MG1655
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| To check that my plasmid works/is correct
 +
|}
 +
 +
|}
|}
__NOTOC__
__NOTOC__

Revision as of 12:58, 5 April 2010


Cluzel Lab Notebook
Daniel Goodman
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Prepping Plasmid for Electroporation

PZE-12-CFP vector from JM Kim

ng/ul 260/280 260/230
56.3 ng 1.92 1.62

Per electroporation, need up to 25 ng of DNA

Doing 4 electroporations, in order to ensure competent cells made last week work correctly.

Media Plasmid Cells Why
LA/Amp PZE-12-CFP My competent MG1655 To get comp cells w/ plasmid
LA PZE-12-CFP My competent MG1655 To check that my competent cells are alive
LA/AMP Amp Plasmid (from Jeff) My competent MG1655 To check that my competent cells transform
LA/AMP PZE-12-CFP Jeff's competent MG1655 To check that my plasmid works/is correct



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