User:Daniel Goodman/Notebook/Cluzel/2010/04/05: Difference between revisions
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Spun down, supernatant removed to ~50ml, resuspend, plated and spun onto correct antibiotic plates w/ LB. Put in 37 degree incubator overnight at 7:45 pm. Will take out tomorrow morning. | |||
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Revision as of 16:47, 5 April 2010
Cluzel Lab Notebook Daniel Goodman |
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Prepping Plasmid for ElectroporationPZE-12-CFP vector from JM Kim
Doing 4 electroporations, in order to ensure competent cells made last week work correctly.
Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book
Spun down, supernatant removed to ~50ml, resuspend, plated and spun onto correct antibiotic plates w/ LB. Put in 37 degree incubator overnight at 7:45 pm. Will take out tomorrow morning. |