User:Daniel Goodman/Notebook/Cluzel/2010/04/02
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| - | == | + | ==Printing spots on glass slide using micrometer stage== |
| - | * | + | * printed from 10.5-11.1 Y axis |
| + | * printing produces reproducible spots, smaller than chip width (need to compute exact diameter) | ||
| + | * printing from higher up might reduce spot size, need to check | ||
| + | * printing repeatedly might reduce spot size | ||
| + | * need to quantify OD:cells per area ratio (might change with stationary vs exponential | ||
| + | * phase and 1 gfp image saved in 4-2 dir on titan | ||
| + | * will transfer to agarose later today: saved slide in petri dish at 12:15 pm | ||
| + | |||
| + | ==Agarose== | ||
| + | * made 1 ml 4% agarose at 12:20 pm | ||
| + | |||
| + | ==Cells== | ||
| + | * grew 1 ml cells with 1 col gfp cells (around 11:45 am); forgot to add amp (1 ul), did so at 12:22 pm | ||
Current revision
Cluzel Lab Notebook Daniel Goodman | Main project page Previous entry Next entry
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Printing spots on glass slide using micrometer stage
Agarose
Cells
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