User:Daniel Goodman/Notebook/Cluzel/2010/03/31: Difference between revisions
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Protocol for Competent Cells | ==Protocol for Competent Cells== | ||
*Grow 250 ml flask, one colony, 50 ml LB at 37 deg for 4 hours (OD must be 0.3-0.4) in shaker (check at ~7pm) - ''OD was 0.83'' | |||
*Spin down 20 mls in 2 50 ml epis at 4 degrees in centrifuge | *Spin down 20 mls in 2 50 ml epis at 4 degrees in centrifuge | ||
**3200 RCF (4000 RPM) 10 minutes 4 degrees | **3200 RCF (4000 RPM) 10 minutes 4 degrees | ||
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* make 20x 50 ul aliquots, put in -80 freezer | * make 20x 50 ul aliquots, put in -80 freezer | ||
==Agarose Chip Prep== | |||
* Put silicon chips in acetone (under fume hood) in glass flask for 5 minutes, heated slightly | |||
* Made 3ml 5% agarose, heat to 80 deg | |||
* Using stationary phase GFP cells, dilute a few colonies in DI water to OD 0.05. | |||
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Latest revision as of 18:10, 31 March 2010
Cluzel Lab Notebook Daniel Goodman |
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Protocol for Competent Cells
Agarose Chip Prep
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