User:Daniel Goodman/Notebook/Cluzel/2010/03/12

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(Autocreate 2010/03/12 Entry for User:Daniel_Goodman/Notebook/Cluzel)
Current revision (14:25, 12 March 2010) (view source)
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==Entry title==
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==Made 5% Agarose==
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* Insert content here...
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* 5 ml LB to .250 g low melt agarose
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* heat for more than 20 min (40?) because of irregular density issues on Wednesday
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==Grow cells==
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* Grow up a few colonies in 4 ml LB with Amp (2:30)
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* take some cells (4 cols) directly off the plate, put in 500 ul DI water, for use in the meantime

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Cluzel Lab Notebook
Daniel Goodman
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Made 5% Agarose

  • 5 ml LB to .250 g low melt agarose
  • heat for more than 20 min (40?) because of irregular density issues on Wednesday


Grow cells

  • Grow up a few colonies in 4 ml LB with Amp (2:30)
  • take some cells (4 cols) directly off the plate, put in 500 ul DI water, for use in the meantime



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