User:Daniel Goodman/Notebook/Cluzel/2010/03/05: Difference between revisions
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===Notes=== | ===Notes=== | ||
*Not enough gel poured in mold, features did not form (but still continued w/ experiment | *Not enough gel poured in mold, features did not form (but still continued w/ experiment) | ||
** In future, try to use more than 108, maybe 110. Removing bubbles (which happens often) often reduces height of agarose blob in mold and ruins features. | |||
*3% - couldn't find any cells, this could be because surface was concave so cells did not touch agarose | *3% - couldn't find any cells, this could be because surface was concave so cells did not touch agarose | ||
*5% - cells found. Large (several screens), toward bottom of mold, so hard to tell extent - ''but clear boundaries were apparent!'' | *5% - cells found. Large (several screens), toward bottom of mold, so hard to tell extent - ''but clear boundaries were apparent!'' |
Latest revision as of 15:46, 5 March 2010
Cluzel Lab Notebook Daniel Goodman |
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Grow cells
Made agarose
Make chip impression in solidified gel
Results: no features found with significant 'push' on 3% gel. Try transferring again (w/ features)
Notes
Placement:
Next time:
While waiting...
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