User:Daniel Goodman/Notebook/Cluzel/2010/03/05: Difference between revisions

From OpenWetWare
< User:Daniel Goodman‎ | Notebook‎ | Cluzel‎ | 2010‎ | 03
Jump to navigationJump to search
No edit summary
Line 13: Line 13:
==Try transferring again (w/ features)==
==Try transferring again (w/ features)==
*Scope access at 4PM
*Scope access at 4PM
*Start gel pouring at 2:30 - Poured 3% at 2:30, waiting for more melting for 5%
*Start gel pouring at 2:30 - Poured 3% at 2:30, poured 5% at 2:50
*Try w/ 3% and 5% agarose


==While waiting...==
==While waiting...==

Revision as of 12:52, 5 March 2010


Cluzel Lab Notebook
Daniel Goodman 		Main project page
Previous entry      Next entry<sitesearch>title=Search this Project</sitesearch>

Grow cells

  • Took a colony from fridge plate of MG1655 w/ GFP, put in 4 mL LB with 4 uL amp in 37°C shaker. (~10:30 AM)

Made agarose

  • Made 5 mL of 5% agarose, and 5 mL of 3% agarose. (~10:40 AM)

Make chip impression in solidified gel

  • Try quick experiment based on results from Wednesday; can we make features on a gel by pressing down?

Results: no features found with significant 'push' on 3% gel.

Try transferring again (w/ features)

  • Scope access at 4PM
  • Start gel pouring at 2:30 - Poured 3% at 2:30, poured 5% at 2:50

While waiting...

  • Play with a few featureless gels, practice flipping & transferring


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Daniel_Goodman/Notebook/Cluzel/2010/03/05&oldid=396796"