User:Daniel Goodman/Notebook/Cluzel/2010/03/03

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(Glycerol Stocks for MG1655 strains)
(Agarose)
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==Experiment==
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I get the stage at ~1:30. I will pour 2 featureless molds and try transferring again, gently, to see if we get a clear spot boundary upon transfer.
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*Put 5% agarose in at 12:56
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*Pour molds into PDMS at 1:15
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*Molds will dry at 1:35
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Revision as of 13:15, 3 March 2010


Cluzel Lab Notebook
Daniel Goodman
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Glycerol Stocks for MG1655 strains

  • 2 culture tubes
  • 4 ml LB in each
  • 4 ul of Amp in one (for GFP strain)
  • took one average-sized colony from each
  • put in 37°C shaker (at ~12:30 pm)

Forgot to take out the cultures on Friday, so doing this again today.

Reminder: Take culture tubes out of shaker at ~6:30 and put stocks in glycerol!

Agarose

  • making 5% agarose (.3 g in 6 mL) for molds that are easier to manipulate and less likely to fracture/distort

Experiment

I get the stage at ~1:30. I will pour 2 featureless molds and try transferring again, gently, to see if we get a clear spot boundary upon transfer.

  • Put 5% agarose in at 12:56
  • Pour molds into PDMS at 1:15
  • Molds will dry at 1:35


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