User:Daniel Goodman/Notebook/Cluzel/2010/03/03

Cluzel Lab Notebook Daniel Goodman

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Glycerol Stocks for MG1655 strains 2 culture tubes 4 ml LB in each 4 ul of Amp in one (for GFP strain) took one average-sized colony from each put in 37°C shaker (at ~12:30 pm) Forgot to take out the cultures on Friday, so doing this again today. Reminder: Take culture tubes out of shaker at ~6:30 and put stocks in glycerol! Agarose making 5% agarose (.3 g in 6 mL) for molds that are easier to manipulate and less likely to fracture/distort

Experiment

I get the stage at ~1:30. I will pour 2 featureless molds and try transferring again, gently, to see if we get a clear spot boundary upon transfer.

• Put 5% agarose in at 12:56
• Pour molds into PDMS at 1:15
• Molds will dry at 1:35