User:Daniel A. Vargas/Notebook/General lab notebook/2015/10/04: Difference between revisions

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Samples were processed today using the Haynes [[Haynes:Luciferase | Luciferase assay]].  
Samples were processed today using the Haynes [[Haynes:Luciferase | Luciferase assay]].  


 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Sample'''
{|{{table}}
| align="center" style="background:#f0f0f0;"|'''cells/20uL'''
| align="center" style="background:#f0f0f0;"|'''Plasmid''
| align="center" style="background:#f0f0f0;"|'''Cells/100uL'''
| align="center" style="background:#f0f0f0;"|'''No dox'''
| align="center" style="background:#f0f0f0;"|'''Luc/Cell'''
| align="center" style="background:#f0f0f0;"|'''48 hours'''
|-
| align="center" style="background:#f0f0f0;"|'''96 hours'''
| KAH60 no dox||16,236||81,180||0.58883551
| align="center" style="background:#f0f0f0;"|'''144hours'''
|-
| align="center" style="background:#f0f0f0;"|'''192 hours'''
| KAH60 48hrs||31,403||157,015||0.13837956
|-
| KAH60 96hrs||20,670||103,350||0.584815352
|-
| KAH60 144hrs||10,081||50,405||0.798650927
|-
| KAH60 192hrs||2,488||12,440||3.7163987
|-
| KAH126 no dox||39,306||196,530||0.235241439
|-
| KAH126 48hrs||23,577||117,885||0.018187216
|-
| KAH126 96hrs||25,146||125,730||0.011089902
|-
| KAH126 144hrs||14,050||70,250||0.008944247
|-
| KAH126 192hrs||7,584||37,920||0.037561533
|-
| No plasmid no dox||31,178||155,890||0.008903714
|-
| No plasmid 48hrs||28,905||144,525||0.015994926
|-
|-
| KAH60||0.58883551||0.13837956||0.584815352|| 0.798650927 ||3.7163987||
| No plasmid 96hrs||30,048||150,240||0.00818682
|-
|-
| KAH126||0.569499877||0.013654746|| 0.013491272||0.012465695||0.1144962||
| No plasmid 144hrs||16,836||84,180||0.013574087
|-
|-
| No plasmid||0.017097807||0.014722585||0.011898||0.022669||0.101875||
| No plasmid 192hrs||14,827||74135||0.017094939
|}
|}
<br>
 
 




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Entry title

Samples were processed today using the Haynes Luciferase assay.

Sample cells/20uL Cells/100uL Luc/Cell
KAH60 no dox 16,236 81,180 0.58883551
KAH60 48hrs 31,403 157,015 0.13837956
KAH60 96hrs 20,670 103,350 0.584815352
KAH60 144hrs 10,081 50,405 0.798650927
KAH60 192hrs 2,488 12,440 3.7163987
KAH126 no dox 39,306 196,530 0.235241439
KAH126 48hrs 23,577 117,885 0.018187216
KAH126 96hrs 25,146 125,730 0.011089902
KAH126 144hrs 14,050 70,250 0.008944247
KAH126 192hrs 7,584 37,920 0.037561533
No plasmid no dox 31,178 155,890 0.008903714
No plasmid 48hrs 28,905 144,525 0.015994926
No plasmid 96hrs 30,048 150,240 0.00818682
No plasmid 144hrs 16,836 84,180 0.013574087
No plasmid 192hrs 14,827 74135 0.017094939




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