User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/04: Difference between revisions
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Master Mix (in 1.5 mL tube) | Master Mix (in 1.5 mL tube) | ||
{| {{table}} | {| {{table}} | ||
| Reagent || Volume || | | Reagent || Volume || x3 | ||
|- | |- | ||
| Oligo Bridge 1 || 2.0 | | Oligo Bridge 1 || 2.0 | ||
|- | |||
| Oligo Bridge 2 || 2.0 | |||
|- | |||
| Oligo Bridge 3 || 2.0 | |||
|- | |- | ||
| 10X Ampligase Buffer || 2.0 | | 10X Ampligase Buffer || 2.0 | ||
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{| | {| | ||
|- | |- | ||
| Reagent || Volume || Rxn1 | | Reagent || Volume || Rxn1 || Rxn2 | ||
|- | |- | ||
| PNK DNA || 10.0 μL | | PNK DNA || 10.0 μL | ||
|} | |||
* Run LCR... | * Run LCR... |
Revision as of 07:12, 4 August 2015
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ASSEMBLY of MV10, ATF2, and Gal4DB/mCherry
LCR Assembly Prep the Bridge Oligos
Overview: Mix double-stranded DNA fragments, treat with PNK to add phosphate groups
Set up the following in PCR tubes:
Master Mix (in 1.5 mL tube)
Add master mix into 10 μL PNK DNA
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