User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/04: Difference between revisions
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'''PNK treatment''' | '''PNK treatment''' | ||
Overview: Mix double-stranded DNA fragments, treat with PNK to add phosphate groups | |||
Procedure: | |||
* Dilute the purified dsDNA to 30 fmol/μL (30 nM) | * Dilute the purified dsDNA to 30 fmol/μL (30 nM) | ||
** The volume of purified DNA (x) you will need to dilute in a final volume of 50 μL = length in bp ÷ measured ng/μL * ''30 fmol/μL * 650 fg/fmol dsDNA ÷ 1,000,000 fg/ng'' * 50 μL final volume. | ** The volume of purified DNA (x) you will need to dilute in a final volume of 50 μL = length in bp ÷ measured ng/μL * ''30 fmol/μL * 650 fg/fmol dsDNA ÷ 1,000,000 fg/ng'' * 50 μL final volume. |
Revision as of 22:04, 3 August 2015
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ASSEMBLY of MV10, ATF2, and Gal4DB/mCherry
LCR Assembly
Procedure:
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