User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/03

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Quality Check of PCR and RED

MV10 digest

Run at 37oC for 15 min
Reagent Volume
MV10 15.00
Xba1 1.00
MBN 1.00
10X FD buffer 3.00
dH2O 10.00
Total 30.00
Reagent Volume Expected:
1. MV10 = 5191,
2. ATF2 = 906
3. ATF2 neg ctrl = 0
4. Gal4db/mCherry = 1170
5. Gal4db/mCherry = 0
 Damaged Gel
10 μL/lane; 1% agarose; Ladder
DNA 3.0 μL
6X BioLabs purple dye 3.0 μL
dH2O 4.0 μL
  10 μL

Qiagen clean-up Results

Plasmid Conc. (ng/µL) St Dev (ng/µL) 260/280
MV10 46.37 1.13 1.75
ATF2 64.350 2.15 1.90
Gal4DB/mCherry 50.873 1.13 1.93


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