User:Daniel A. Vargas/Notebook/General lab notebook/2015/08/03

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Quality Check of PCR and RED

Reagent Volume Expected:
1. MV10 = 5191,
2. ATF2 = 906
3. ATF2 neg ctrl = 0
4. Gal4db/mCherry = 1170
5. Gal4db/mCherry = 0
 Damaged Gel
10 μL/lane; 1% agarose; Ladder
DNA 3.0 μL
6X BioLabs purple dye 3.0 μL
dH2O 4.0 μL
  10 μL

Qiagen clean-up Results

Plasmid Conc. (ng/µL) St Dev (ng/µL) 260/280
MV10 46.37 1.13 1.75
ATF2 64.350 2.15 1.90
Gal4DB/mCherry 50.873 1.13 1.93


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