User:Daniel A. Vargas/Notebook/General lab notebook/2015/06/29

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PCR amplification of chromatin remodeling

Reagent Volume Expected:
1. ANM5 = 1911,
2. MYB = 159,1764
3. Carm1 = 1827
4. SETD7 = 459,642
5. KMT2E = 1176,4401
6. SETD1A = 420,4704
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 1.0 μL
1X GoTaq 12.5
F primer 1.0
R Primer 1.0
dH2O 9.5
  25 μL

Transforming P300 plasmids

Transformation protocol

Sample ID Expected Observed colonies
PBS P300 Growth NA 2.09
NHA P300 Growth NA 2.07
CHA P300 Growth NA 2.09

Samples were transformed using the Heat shock transformation rather than the rapid transformation protocol.


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