User:Daniel A. Colombo/Notebook/Biology 210 at AU

Lab Journal Exercise 4

Within our transect we did not much space in which to choose from for plants. Many of our plants came from the garden boxes where certain plants were supposed to be growing (ie. lettuce). A few of the plants we picked up were growing through the wood chips, and were more like weeds. It was hard to find a variety of plants due to the wood chips and the fact that our transect is a garden post-harvest in the winter.

Because I could not find a way to cleanly upload the characterization table, I formatted it into sentence form and added it here.

Plant #1 was a big leaf, pulled from the lettuce growing box. it had longitudinal vascularization with no particular pattern. It had a waxy coating along with a simple structure. It was also a gymnosperm. We classfied it as a dicot. Plant #2 was a smaller plant pulled from the wood chips with parallel vascularization. The plant grew straight up and was also a gymnosperm. We classified it as a monocot. Plant #3 it was clover-shaped and pulled from the wood chips. It had netlike vasculrizatiuon and a trap root. It was a gymnosperm and a dicot. Plant #4 was found in the wood chips again. It had a small stem with a bigger leaf. It had netlike vacuolization. It was a also a gymnosperm and a dicot. Plant#5 was found in the plant box and had a waxy coating. Parallel vascularization. This plant was alos a gymnosperm and a monocot.

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Journal Exercise 3

The water in our Hay infusion culture has evaporated slightly and with less water all of the material inside of the culture are getting closer together and the smell is growing worse. As the water evaporates there is more oxygen present for certain sediment and more of the culture is exposed to the air. I believe archaea species will not have grown on our agar plates because the bacterial species are more abundant and will have pushed out any archaean species.

With no tet on the plate there was more colony growth across all 8 agar plates. The more diluted the sample was the less bacterial growth there was in all cases. The tet was effective in inhibiting the growth of bacterial colonies from our Hay infusion sample. Tet does a very good job of prohibiting bacterial growth, in every scenario the colony growth on the plate with tet with exponentially lower than without tet. There are no species that were present on our plates that were resistant to the tetracycline. 

In our gram tests, we found only one of our four samples to be gram positive otherwise there were all negative. It was difficult to see the cells because they were so small however in all four samples there was motility, you could slighty see the movement of bacteria in the slide, it just looked like small beads were vibrating.

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