User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/29: Difference between revisions
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#Run a 0-300mM NaCl in Tris gradient over 30 minutes and collect fractions | #Run a 0-300mM NaCl in Tris gradient over 30 minutes and collect fractions | ||
#Combine desired fractions with added 20mM pH 8.3 Tris buffer | #Combine desired fractions with added 20mM pH 8.3 Tris buffer | ||
#Concentrate solution in centrifuge | |||
#Inject solution into Q-Sepharose column | |||
##Collect elute during injection | |||
#Run a 0-300mM NaCl in Tris gradient over 60 minutes and collect fractions | |||
#Combine desired fractions with added 10mM pH 7.2 Phosphate buffer | |||
#Concentrate solution in centrifuge | #Concentrate solution in centrifuge | ||
Revision as of 06:24, 30 July 2014
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Preparing sample
Purification
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