User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/25: Difference between revisions
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#The tubes were placed in the freezer until further purification next week | #The tubes were placed in the freezer until further purification next week | ||
==To do next week== | |||
#Concentrate protein from desalting column | |||
#Run through Q-column w/ gradient 0-30% NaCl 10 minutes and collect fractions | |||
#concentrate elute from q-column | |||
#Run through Q-column w/ gradient 0-30% NaCl 30 minutes and collect fractions | |||
#concentrate elute from q-column | |||
#Run through Q-column w/ gradient 0-30% NaCl 1 hour and collect fractions | |||
#concentrate elute from q-column | |||
#run gel | |||
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Revision as of 12:29, 25 July 2014
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Procedure
To do next week
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