User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/24: Difference between revisions
From OpenWetWare
No edit summary |
(fix raw html notebook nav) |
||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 11: | Line 11: | ||
#A 1/100th dilution of this solution was prepared and a UV-Vis spectra was taken of it | #A 1/100th dilution of this solution was prepared and a UV-Vis spectra was taken of it | ||
#Using Beer's Law the concentration of protein was calculated | #Using Beer's Law the concentration of protein was calculated | ||
##Molar Absorptivity at 415nm: 128,000 | |||
##Absorbance at 415nm : 0.036 | |||
##The concentration of protein (not in diluted sample) was calculated to be 28.125uM | ##The concentration of protein (not in diluted sample) was calculated to be 28.125uM | ||
##This corresponds to 2.53125 * 10^-7 moles of Hemoglobin | ##This corresponds to 2.53125 * 10^-7 moles of Hemoglobin |
Latest revision as of 00:07, 27 September 2017
Project name | Main project page Previous entry Next entry |
Procedure
|