User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/23
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SDS-PAGE
Gel ElectrophoresisWe will be using a Bio-Rad Mini Protean system with pre-cast Mini Protean TGX gels. The manual for this system can be found here We will be running SDS-PAGE followed by gel development with Coomassie Blue staining. Below is a short description of how we will proceed. Please refer to the manual for more detailed instructions
Stock Solutions
-1mg BSA and 1mg Myoglobin in 1mL water -Solution was diluted to 1/10 to be used in gel
-14.4g Glycine, 1g SDS, 3g Tris in 100mL H2O -Solution was diluted to 1L with water
-40 Methanol, 10% Acetic Acid, 50% Water
-90% water, 10$ Acetic Acid and .0025% Commassie Brilliant Blue
-90% Water and 10% Acetic Acid ResultsThe corner with the nick is the top corner above lane 1. The gel showed that the elutes from the SP-column were purified from the original solution but not as pure as desired. The elutes were combined and concentrated in a centrifugal device at 3680rpm until the final volume was ~10mL
|