User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/22: Difference between revisions
From OpenWetWare
(Autocreate 2014/07/22 Entry for User:Daniel-Mario_Larco/Notebook/AU_Photosynthesis_Lab) |
(fix raw html notebook nav) |
||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Q-Sepharose column== | ||
The remaining protein solution was placed in the fridge with 50mM Tris buffer at pH 8.3 | |||
#The Q-sepharose column was attached to the FPLC | |||
#The protein solution was loaded in 50mL at a time | |||
##the elute was collected during injection | |||
#After each 50mL, a gradient of 0-160mM NaCl was run in Tris pH 8.3 | |||
##Fractions were collected during the gradient | |||
###For the first 50mL, fractions 4-10 were collected | |||
###For the second 50mL, fractions 15-21 were collected | |||
#Fractions 1-3, 11-14 and 22-23 were collected in a separate tube for testing | |||
#Between each injection, the column was cleaning with 1M NaCL and reequilibrated with 20mM Tris at pH8.3 | |||
#The column was cleaned and equilibrated with 20% Ethanol solution and stored | |||
==SP-Sepharose column== | |||
#The SP-Sepharose column was installed | |||
#The filter to the FPLC was also changed | |||
#The two sets of fractions were concentrated at 3680rpm at 4C in centrifugal devices with pH7.2 phosphate buffer 4 times in order to change the pH | |||
#The SP-Sepharose column was equilibrated with pH7.2 phosphate buffer | |||
#The two sets were loaded on the column and the run-through was collected | |||
#The column was cleaned using 1M NaCl in tris pH8.3 | |||
##the elute was collected for testing purposes | |||
Latest revision as of 00:07, 27 September 2017
Project name | Main project page Previous entry Next entry |
Q-Sepharose columnThe remaining protein solution was placed in the fridge with 50mM Tris buffer at pH 8.3
SP-Sepharose column
|