User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/14: Difference between revisions

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##1/10 dilution of purified protein from dialysis over the weekend
##1/10 dilution of purified protein from dialysis over the weekend
##1/100 dilution of purified protein from dialysis over the weekend
##1/100 dilution of purified protein from dialysis over the weekend
##Empty
##1/10 dilution of centrifuged purified protein from dialysis over the weekend
##Myoglobin and BSA ladder
##Myoglobin and BSA ladder


Revision as of 06:49, 14 July 2014

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  1. Our protein solution was collected from the cold room and transferred into a 50mL falcon tube labeled Purified Hb pH7, 7/14/14
  2. We will run a gel with the solution and past solution to check purity
  3. The gel will be set up in the following order
    1. Myoglobin and BSA ladder
    2. Empty
    3. 1/10 dilution of Protein in pH8.3 tris (solution that was injected into Q-Sepharose column)
    4. Elute during injection of Protein in pH8.3 tris into Q-Sepharose column
    5. Elute during cleaning Q-Sepharose column containing Protein in pH8.3 tris with 1M NaCl
    6. Elute from SP-Sepharose column in phosphate buffer pH7.2 during injection (from fractions collected from Q-Sepharose column)
    7. Empty
    8. 1/10 dilution of Ammonium sulfate treatment pellet dissolved in 50mM Tris
    9. 1/10 dilution of purified protein from dialysis over the weekend
    10. 1/100 dilution of purified protein from dialysis over the weekend
    11. 1/10 dilution of centrifuged purified protein from dialysis over the weekend
    12. Myoglobin and BSA ladder



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