User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/07/10: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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#A small amount of 5mM Tris buffer was used to collect remaining traces of cells from the centrifuge tubes | #A small amount of 5mM Tris buffer was used to collect remaining traces of cells from the centrifuge tubes | ||
#The pellet was then sonified | #The pellet was then sonified | ||
##30 seconds | ##30 seconds sonification followed by 30 seconds on ice until the pellet was loose and liquid | ||
#Once liquefied, the black supernatant was put back in the tubes and the solution was centrifuged at 20,000rpm four 3 hours at 4C | #Once liquefied, the black supernatant was put back in the tubes and the solution was centrifuged at 20,000rpm four 3 hours at 4C | ||
##The tubes were spun for an additional 30 minutes under the same conditions in order to prepare the materials for the next step | ##The tubes were spun for an additional 30 minutes under the same conditions in order to prepare the materials for the next step |
Latest revision as of 00:06, 27 September 2017
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Cell and Protein Extraction
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