User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/04/02: Difference between revisions
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==Protein Expression== | |||
#Cells were placed in each of the 25mL Erlenmeyer flasks and left to grow overnight in a shaking incubator at 37C and 200rpm | |||
#Each of these solutions was transferred to a 1L broth solution with 1mL of ampicilin | |||
#The flasks were left to incubate for 4 hours at 37C on the shaking incubator at 150rpm | |||
#After 4 hours 1ml of Fe-protoporphyrin IX was added to each flask and the temperature was reduced to 30C and left to incubate for another 4 hours | |||
##The Fe-protoporphyrin IX was made by dissolving 40mg of Fe-protoporphyrin IX in 1mL of DMSO | |||
#After four hours, the flasks were placed in a cold room over night | |||
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Revision as of 07:53, 10 April 2014
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Protein Expression
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