User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/02/19: Difference between revisions

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==Expression of Myoglobin==
==Expression of Myoglobin==
*The cells that grew on the plate were placed in 100mL of water and 100uL ampicilin and spun overnight. x4
*The cells that grew on the plate were placed in 100mL of water and 100uL ampicilin and spun overnight. x4
*The spun solutions we placed in the 1L Broth flask along with a mL of ampicilin and 1mL of expression factor
*The spun solutions we placed in the 1L Broth flask along with a mL of ampicilin and 1mL of 0.1mM δ-aminolevulinic acid (ALA)
*The solutions were again spun overnight.
*The solutions were again spun overnight.



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Expression of Myoglobin

  • The cells that grew on the plate were placed in 100mL of water and 100uL ampicilin and spun overnight. x4
  • The spun solutions we placed in the 1L Broth flask along with a mL of ampicilin and 1mL of 0.1mM δ-aminolevulinic acid (ALA)
  • The solutions were again spun overnight.