User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/01/15: Difference between revisions
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== | ==Preparing Broth and Agar== | ||
==Objective== | |||
During this lab session, solutions of LB/Agar and LB Broth were prepared. A solution of Ampicilin was prepared to be added to the broth and agar. | |||
==Procedure== | |||
#The correct concentration for dissolving LB Broth Media into water is 25g per liter of water. | |||
The correct concentration for dissolving granulated Agar into water is 20g per liter of water. | |||
- For the LB/Agar solution. | |||
2.51g of LB Broth Media and 2.00g Difco (TM) granulated Agar were dissolved in 100mL of water | |||
- For the LB Broth solution, | |||
1.25g of LB Broth Media were dissolved into 50mL of water | |||
#In order to prepare the ampicilin solution, 100mg of Ampicilin Sodium Salt were dissolved into 1mL of sterilized water. | |||
This solution was prepared in a sterilized eppendorf tube using sterile equipment. | |||
Revision as of 08:24, 15 January 2014
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Preparing Broth and AgarObjectiveDuring this lab session, solutions of LB/Agar and LB Broth were prepared. A solution of Ampicilin was prepared to be added to the broth and agar. Procedure
The correct concentration for dissolving granulated Agar into water is 20g per liter of water. - For the LB/Agar solution. 2.51g of LB Broth Media and 2.00g Difco (TM) granulated Agar were dissolved in 100mL of water - For the LB Broth solution, 1.25g of LB Broth Media were dissolved into 50mL of water
This solution was prepared in a sterilized eppendorf tube using sterile equipment.
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