# User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/01/15

(Difference between revisions)
 Revision as of 10:16, 15 January 2014 (view source) (Autocreate 2014/01/15 Entry for User:Daniel-Mario_Larco/Notebook/AU_Photosynthesis_Lab)← Previous diff Revision as of 10:24, 15 January 2014 (view source)Next diff → Line 6: Line 6: | colspan="2"| | colspan="2"| - ==Entry title== + ==Preparing Broth and Agar== - * Insert content here... + + ==Objective== + During this lab session, solutions of LB/Agar and LB Broth were prepared. A solution of Ampicilin was prepared to be added to the broth and agar. + + ==Procedure== + #The correct concentration for dissolving LB Broth Media into water is 25g per liter of water. + The correct concentration for dissolving granulated Agar into water is 20g per liter of water. + - For the LB/Agar solution. + 2.51g of LB Broth Media and 2.00g Difco (TM) granulated Agar were dissolved in 100mL of water + - For the LB Broth solution, + 1.25g of LB Broth Media were dissolved into 50mL of water + #In order to prepare the ampicilin solution, 100mg of Ampicilin Sodium Salt were dissolved into 1mL of sterilized water. + This solution was prepared in a sterilized eppendorf tube using sterile equipment. +

## Revision as of 10:24, 15 January 2014

Project name Main project page
Next entry

## Objective

During this lab session, solutions of LB/Agar and LB Broth were prepared. A solution of Ampicilin was prepared to be added to the broth and agar.

## Procedure

1. The correct concentration for dissolving LB Broth Media into water is 25g per liter of water.
```The correct concentration for dissolving granulated Agar into water is 20g per liter of water.
- For the LB/Agar solution.
2.51g of LB Broth Media and 2.00g Difco (TM) granulated Agar were dissolved in 100mL of water
- For the LB Broth solution,
1.25g of LB Broth Media were dissolved into 50mL of water
```
1. In order to prepare the ampicilin solution, 100mg of Ampicilin Sodium Salt were dissolved into 1mL of sterilized water.

This solution was prepared in a sterilized eppendorf tube using sterile equipment.