User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/02/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Procedures== | ||
# Ocean Optics was used to perform continuous absorbance spectra in order to observe hemoglobin activity | |||
##Solutions were prepared to run the Ocean Optics tests | |||
### 3.0285mg of Tris were dissolved in 0.5L H2O, and pH was adjusted to 7.5 using 1N Hydrochloric acid | |||
### 870.5mg of Sodium Dithionite were dissolved in 500mL of Tris buffer | |||
# Both solutions were debased by placing a Nitrogen line in the solutions for a few hours | |||
# The data was collected then analyzed | |||
==Data== | |||
Graph showing how pure hemoglobin changes over time in the presence of Sodium Dithionite | |||
[[Image:Hemoglobin control ocean.png|500px]] | |||
- This graph will be used as a basis for comparison of the nano particles coated with Hemoglobin | |||
Graph showing absorbance changes in alpha hemoglobin nanoparticles over time | |||
[[Image:Alpha Hemoglobin.png|500px]] | |||
- In this graph we notice that the peak at around 400nm decreases but no new peak is formed, which suggests that something isn't right. | |||
Graph showing absorbance changes in beta hemoglobin nanoparticles over time | |||
[[Image:Beta hemoglobin ocean.png|500px]] | |||
- In this graph we can see activity in the Beta-Method Hemoglobin coated AuNPs | |||
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Latest revision as of 23:54, 26 September 2017
Project name | Main project page Previous entry Next entry |
Procedures
DataGraph showing how pure hemoglobin changes over time in the presence of Sodium Dithionite - This graph will be used as a basis for comparison of the nano particles coated with Hemoglobin
- In this graph we notice that the peak at around 400nm decreases but no new peak is formed, which suggests that something isn't right.
- In this graph we can see activity in the Beta-Method Hemoglobin coated AuNPs
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