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<font size="4">Damian C. Ekiert</font size>
<font size="4">Damian C. Ekiert</font size>


<font size="2">The Skaggs Institute for Chemical Biology<br>
<font size="2">Damon Runyon Post-Doctoral Fellow<br>
The Scripps Research Institute<br>
Department of Microbiology and Immunology<br>
10550 N. Torrey Pines Rd.<br>
The University of California, San Francisco<br>
La Jolla, CA 92037<br>
600 16th St.<br>
 
Genentech Hall Rm. 376N
dcekiert at scripps dot edu
San Francisco, CA 94143<br>
415-476-5159<br>
damian.ekiert at ucsf dot edu


</font size>
</font size>
|}
|}


Hello, I am a graduate student in the [http://www.scripps.edu/mb/wilson/ Wilson Lab] at [http://www.scripps.edu The Scripps Research Institute].
I am a post-doctoral fellow in the [http://coxlab.ucsf.edu/COX_WEB_SITE/Welcome.html Jeff Cox's Lab] at [http://www.ucsf.edu The University of California, San Francisco].




== Research Interests ==
== Research Interests ==
<blockquote>  
<blockquote>  
*Structure-function studies of hemagglutinin receptor specificity (H5N1 avian viruses, 2,3 vs. 2,6 sialic acids)
*The adaptive immune response against Mycobacterium tuberculosis
*Directed evolution/Display technology
*Antigenic variation and immune evasion
*Synthetic biology
*Vaccine design for pathogens of global importance
</blockquote>
</blockquote>


== Education==
== Education==
<blockquote>
<blockquote>
B.A. in Biology, [http://www.uchicago.edu The University of Chicago] <br>
*Present, Post-Doctoral Fellow, [http://www.ucsf.edu The University of California, San Francisco]
*2011, Ph.D.,  [http://www.scripps.edu|The Scripps Research Institute]<br>
*2005, A.B. in Biology, [http://www.uchicago.edu The University of Chicago] <br>
</blockquote>
</blockquote>


== Past Lab Work==
==Research Experience==
<blockquote>
<blockquote>
*Undergraduate honors thesis in Bernard Strauss's Lab, where we were investigating the coordination of DNA replication and cell division in ''E. coli''. <br>
*2012-Present: '''Prof. Jeffery Cox Lab''', Post-Doc, investigation of antigenic variation and immune evasion in TB.
*Year abroad at the University of Paris, working in the labs of Miroslav Radman and Masamichi Kohiyama on mismatch-stimulated killing and the localization of replication forks in ''E. coli''.
*2007-2012: '''Prof. Ian A. Wilson Lab''', thesis, structural characterization of broadly neutralizing antibodies against the influenza A virus.
*A "gap-year" in Michael Glotzer's Lab working on cytokinesis in mammalian cells.
*2006-2007: '''Prof. Carlos Barbas Lab''', graduate rotation, directed evolution of site specific recombinases for genome engineering and gene therapy.
<br>[http://www.example.com link title]
*2005-2006: '''Prof. Michael Glotzer Lab''' (gap year) working on cytokinesis in mammalian cells.
*2003-2004: '''Prof. Miroslav Radman''' (Université Paris Descartes) and '''Prof. Masamichi Kohiyama''' (Université Pierre et Marie Curie) groups during year abroad in Paris, working on mismatch-stimulated killing and the localization of replication forks in ''E. coli''.
*2003-2005: '''Prof. Bernard Strauss Lab''' (University of Chicago), undergraduate honors thesis, investigating the coordination of DNA replication and cell division in ''E. coli''.
</blockquote>
</blockquote>


== Publications ==
== Publications ==
<blockquote>
<blockquote>
<biblio>
*Nycholat CM, McBride R, '''Ekiert DC''', Xu R, Rangarajan J, Peng W, Razi N, Gilbert M, Wakarchuk W, Wilson IA, Paulson JC.
#coolpmid pmid=16352851
:[http://www.ncbi.nlm.nih.gov/pubmed/22505324 ''Recognition of sialylated poly-N-acetyllactosamine chains on N- and O-linked glycans by human and avian influenza A virus hemagglutinins''.]
#coolpmid pmid=15805529
:'''Angew Chem Int Ed Engl'''. 2012 May 14;51(20):4860-3.
#coolpmid pmid=15028700
:PMID: 22505324
</biblio>
 
*'''Ekiert DC''', Wilson IA.
:[http://www.ncbi.nlm.nih.gov/pubmed/22482710 ''Broadly neutralizing antibodies against influenza virus and prospects for universal therapies''.]
:'''Curr Opin Virol'''. 2012 Apr;2(2):134-41.
:PMID: 22482710
 
*'''<nowiki>*</nowiki>Ekiert DC''', <nowiki>*</nowiki>Friesen RH, Bhabha G, Kwaks T, Jongeneelen M, Yu W, Ophorst C, Cox F, Korse HJ, Brandenburg B, Vogels R, Brakenhoff JP, Kompier R, Koldijk MH, Cornelissen LA, Poon LL, Peiris M, Koudstaal W, Wilson IA, Goudsmit J.
:[http://www.ncbi.nlm.nih.gov/pubmed/21737702 ''A highly conserved neutralizing epitope on group 2 influenza A viruses''.]
:'''Science'''. 2011 Aug 12;333(6044):843-50.
:PMID: 21737702
 
*<nowiki>*</nowiki>Fleishman SJ, <nowiki>*</nowiki>Whitehead TA, '''<nowiki>*</nowiki>Ekiert DC''', Dreyfus C, Corn JE, Strauch EM, Wilson IA, Baker D.
:[http://www.ncbi.nlm.nih.gov/pubmed/21566186 ''Computational design of proteins targeting the conserved stem region of influenza hemagglutinin''.]
:'''Science'''. 2011 May 13;332(6031):816-21.
:PMID: 21566186
 
*Bhabha G, Lee J, '''Ekiert DC''', Gam J, Wilson IA, Dyson HJ, Benkovic SJ, Wright PE.
:[http://www.ncbi.nlm.nih.gov/pubmed/21474759 ''A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis''.]
:'''Science'''. 2011 Apr 8;332(6026):234-8.
:PMID: 21474759
 
*<nowiki>*</nowiki>Krause JC, '''<nowiki>*</nowiki>Ekiert DC''', Tumpey TM, Smith PB, Wilson IA, Crowe JE Jr.
:[http://www.ncbi.nlm.nih.gov/pubmed/21304166 ''An insertion mutation that distorts antibody binding site architecture enhances function of a human antibody''.]
:'''MBio'''. 2011 Feb 8;2(1):e00345-10.
:PMID: 21304166
 
*Wang TT, Tan GS, Hai R, Pica N, Ngai L, '''Ekiert DC''', Wilson IA, García-Sastre A, Moran TM, Palese P.
:[http://www.ncbi.nlm.nih.gov/pubmed/20956293 ''Vaccination with a synthetic peptide from the influenza virus hemagglutinin provides protection against distinct viral subtypes''.]
:'''Proc Natl Acad Sci U S A'''. 2010 Nov 2;107(44):18979-84.
:PMID: 20956293
 
*'''Xu R <nowiki>*</nowiki>''', '''Ekiert DC <nowiki>*</nowiki>''', Krause JC, Hai R, Crowe JE Jr, Wilson IA.
:[http://www.ncbi.nlm.nih.gov/pubmed/20339031 ''Structural basis of preexisting immunity to the 2009 H1N1 pandemic influenza virus''.]
:'''Science'''. 2010 Apr 16;328(5976):357-60.
:PMID: 20339031
 
*'''Ekiert DC''', Bhabha G, Elsliger MA, Friesen RH, Jongeneelen M, Throsby M, Goudsmit J, Wilson IA.
:[http://www.ncbi.nlm.nih.gov/pubmed/19251591 ''Antibody recognition of a highly conserved influenza virus epitope''.]
:'''Science'''. 2009 Apr 10;324(5924):246-51.
:PMID: 19251591
 
*Simon GC, Schonteich E, Wu CC, Piekny A, '''Ekiert D''', Yu X, Gould GW, Glotzer M, Prekeris R.
:[http://www.ncbi.nlm.nih.gov/pubmed/18511905 ''Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis''.]
:'''EMBO J'''. 2008 Jul 9;27(13):1791-803.
:PMID: 18511905
 
*Matic I, '''Ekiert D''', Radman M, Kohiyama M.
:[http://www.ncbi.nlm.nih.gov/pubmed/16352851 ''Generation of DNA-free Escherichia coli cells by 2-aminopurine requires mismatch repair and nonmethylated DNA''.]
:'''J Bacteriol'''. 2006 Jan;188(1):339-42.
:PMID: 16352851
 
*Strauss B, Kelly K, '''Ekiert D'''.
:[http://www.ncbi.nlm.nih.gov/pubmed/15805529 ''Cytochrome oxidase deficiency protects Escherichia coli from cell death but not from filamentation due to thymine deficiency or DNA polymerase inactivation''.]
:'''J Bacteriol'''. 2005 Apr;187(8):2827-35.
:PMID: 15805529
 
*Strauss B, Kelly K, Dincman T, '''Ekiert D''', Biesieda T, Song R.
:[http://www.ncbi.nlm.nih.gov/pubmed/15028700 ''Cell death in Escherichia coli dnaE(Ts) mutants incubated at a nonpermissive temperature is prevented by mutation in the cydA gene''.]
:'''J Bacteriol'''. 2004 Apr;186(7):2147-55.
:PMID: 15028700
</blockquote>
</blockquote>
[[Image:Hemagglutinin_Conformational_Changes_Membrane_Fusion.mov]]

Revision as of 10:30, 22 February 2013

Damian C. Ekiert

Damon Runyon Post-Doctoral Fellow
Department of Microbiology and Immunology
The University of California, San Francisco
600 16th St.
Genentech Hall Rm. 376N San Francisco, CA 94143
415-476-5159
damian.ekiert at ucsf dot edu

I am a post-doctoral fellow in the Jeff Cox's Lab at The University of California, San Francisco.


Research Interests

  • The adaptive immune response against Mycobacterium tuberculosis
  • Antigenic variation and immune evasion
  • Vaccine design for pathogens of global importance

Education

Research Experience

  • 2012-Present: Prof. Jeffery Cox Lab, Post-Doc, investigation of antigenic variation and immune evasion in TB.
  • 2007-2012: Prof. Ian A. Wilson Lab, thesis, structural characterization of broadly neutralizing antibodies against the influenza A virus.
  • 2006-2007: Prof. Carlos Barbas Lab, graduate rotation, directed evolution of site specific recombinases for genome engineering and gene therapy.
  • 2005-2006: Prof. Michael Glotzer Lab (gap year) working on cytokinesis in mammalian cells.
  • 2003-2004: Prof. Miroslav Radman (Université Paris Descartes) and Prof. Masamichi Kohiyama (Université Pierre et Marie Curie) groups during year abroad in Paris, working on mismatch-stimulated killing and the localization of replication forks in E. coli.
  • 2003-2005: Prof. Bernard Strauss Lab (University of Chicago), undergraduate honors thesis, investigating the coordination of DNA replication and cell division in E. coli.

Publications

  • Nycholat CM, McBride R, Ekiert DC, Xu R, Rangarajan J, Peng W, Razi N, Gilbert M, Wakarchuk W, Wilson IA, Paulson JC.
Recognition of sialylated poly-N-acetyllactosamine chains on N- and O-linked glycans by human and avian influenza A virus hemagglutinins.
Angew Chem Int Ed Engl. 2012 May 14;51(20):4860-3.
PMID: 22505324
  • Ekiert DC, Wilson IA.
Broadly neutralizing antibodies against influenza virus and prospects for universal therapies.
Curr Opin Virol. 2012 Apr;2(2):134-41.
PMID: 22482710
  • *Ekiert DC, *Friesen RH, Bhabha G, Kwaks T, Jongeneelen M, Yu W, Ophorst C, Cox F, Korse HJ, Brandenburg B, Vogels R, Brakenhoff JP, Kompier R, Koldijk MH, Cornelissen LA, Poon LL, Peiris M, Koudstaal W, Wilson IA, Goudsmit J.
A highly conserved neutralizing epitope on group 2 influenza A viruses.
Science. 2011 Aug 12;333(6044):843-50.
PMID: 21737702
  • *Fleishman SJ, *Whitehead TA, *Ekiert DC, Dreyfus C, Corn JE, Strauch EM, Wilson IA, Baker D.
Computational design of proteins targeting the conserved stem region of influenza hemagglutinin.
Science. 2011 May 13;332(6031):816-21.
PMID: 21566186
  • Bhabha G, Lee J, Ekiert DC, Gam J, Wilson IA, Dyson HJ, Benkovic SJ, Wright PE.
A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis.
Science. 2011 Apr 8;332(6026):234-8.
PMID: 21474759
  • *Krause JC, *Ekiert DC, Tumpey TM, Smith PB, Wilson IA, Crowe JE Jr.
An insertion mutation that distorts antibody binding site architecture enhances function of a human antibody.
MBio. 2011 Feb 8;2(1):e00345-10.
PMID: 21304166
  • Wang TT, Tan GS, Hai R, Pica N, Ngai L, Ekiert DC, Wilson IA, García-Sastre A, Moran TM, Palese P.
Vaccination with a synthetic peptide from the influenza virus hemagglutinin provides protection against distinct viral subtypes.
Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18979-84.
PMID: 20956293
  • Xu R *, Ekiert DC *, Krause JC, Hai R, Crowe JE Jr, Wilson IA.
Structural basis of preexisting immunity to the 2009 H1N1 pandemic influenza virus.
Science. 2010 Apr 16;328(5976):357-60.
PMID: 20339031
  • Ekiert DC, Bhabha G, Elsliger MA, Friesen RH, Jongeneelen M, Throsby M, Goudsmit J, Wilson IA.
Antibody recognition of a highly conserved influenza virus epitope.
Science. 2009 Apr 10;324(5924):246-51.
PMID: 19251591
  • Simon GC, Schonteich E, Wu CC, Piekny A, Ekiert D, Yu X, Gould GW, Glotzer M, Prekeris R.
Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis.
EMBO J. 2008 Jul 9;27(13):1791-803.
PMID: 18511905
  • Matic I, Ekiert D, Radman M, Kohiyama M.
Generation of DNA-free Escherichia coli cells by 2-aminopurine requires mismatch repair and nonmethylated DNA.
J Bacteriol. 2006 Jan;188(1):339-42.
PMID: 16352851
  • Strauss B, Kelly K, Ekiert D.
Cytochrome oxidase deficiency protects Escherichia coli from cell death but not from filamentation due to thymine deficiency or DNA polymerase inactivation.
J Bacteriol. 2005 Apr;187(8):2827-35.
PMID: 15805529
  • Strauss B, Kelly K, Dincman T, Ekiert D, Biesieda T, Song R.
Cell death in Escherichia coli dnaE(Ts) mutants incubated at a nonpermissive temperature is prevented by mutation in the cydA gene.
J Bacteriol. 2004 Apr;186(7):2147-55.
PMID: 15028700

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