User:Dadrummond

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(To do)
(Protocols under construction)
Line 20: Line 20:
Detection of soluble and insoluble proteins: [[Drummond:Solubility]]
Detection of soluble and insoluble proteins: [[Drummond:Solubility]]
 +
Buffers for lysis: [[Drummond:Lysis]]
Buffers for lysis: [[Drummond:Lysis]]
 +
Protein slot-blot: [[Drummond:Protein Slot Blot Protocol]]
Protein slot-blot: [[Drummond:Protein Slot Blot Protocol]]
 +
 +
Ubiquitin western: [[Drummond:Ubiquitin Western]]
Test page for CSS columns: [[Drummond:Columns]]
Test page for CSS columns: [[Drummond:Columns]]

Revision as of 09:22, 22 July 2007

This is Allan's page. Drummond

To do

Controls.

  • HXK2 using native antibody
    • Tests whether results depend on the tag or not
  • Complement CDC19 deletion using acceptor-bracketed allele
    • Tests whether sandwiched proteins are functional
  • Optimize soluble/insoluble extraction conditions
    • Glass bead lysis
    • Chemical lysis

Templates

Template:Drummond_Top

Template:Drummond_Bottom

Template:Drummond_Body

Protocols under construction

Detection of soluble and insoluble proteins: Drummond:Solubility

Buffers for lysis: Drummond:Lysis

Protein slot-blot: Drummond:Protein Slot Blot Protocol

Ubiquitin western: Drummond:Ubiquitin Western

Test page for CSS columns: Drummond:Columns

Personal tools